ABSTRACT
This study was carried out to examine the hepatoprotective effect of ethylacetate extract of tomatoes and methanol extract of onions on the biochemical changes induced by feed formulated with 15% roasted beef and 85% rats pellets. Doses of 500 mg/kg body weight of both extracts were administered orally. The hepatic activities of aspartate aminotransferase (AST), alanine amino-transferase (ALT) and alkaline phosphatase (ALP) were examined, also concentrations of total protein, albumin and globulin were monitored in the animals. The effects of feeding and treatment on oxidative stress parameters (CAT, SOD, GPx, Glutathione and MDA) were determined. The changes observed were discussed.
ABSTRACT
This Study investigated the protective effect of Gongronema latifolium supplemented diet against acute hepatotoxicity induced by crude petroleum oil on male rats. Oral exposure of rats to crude petroleum significantly decreased (P<0.05) the activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD). Significant decrease in concentrations of glutathione and increase in malondialdehyde (MDA) was observed also, but catalase activity decreased nonsignificantly. Activities of these oxidative stress enzymes and concentrations of glutathione and MDA in rats exposed to crude petroleum and fed simultaneously with 20% G. latifolium supplemented diet were close to those obtained in control rats. Fluctuations in liver function parameters and serum lipid profile were observed. These results indicate that consumption of G. latifolium supplemented diet has protective effect against crude oil induced liver tissue damage.
ABSTRACT
The effect of Gongronema latifolium and Ocimum gratissimum supplemented diet on diesel petroleum induced liver damage in rats was investigated. Rats were acclimatized for two weeks and divided into three groups. Oxidative stress was induced with diesel petroleum (4 ml/kg body weight) at alternate days for seven days in group II and III rats. Group II rats were fed the supplemented diet while Group I and III fed on normal rat pellet only. After one week of feeding, the fluctuations in the activities of antioxidant enzymes - superoxide dismutase and glutathione peroxidase and concentrations of glutathione and malondialdehyde (MDA) indicated stress in the liver of the rats exposed to diesel. Significant increase (P<0.05) in serum concentration of Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) and other liver damage markers was observed in group III rats. Serum lipid profile was also studied. Feeding with the supplemented diet showed significant reduction (P<0.05) in the activities of ALT and AST and in the concentration of MDA. The activities of some antioxidant enzymes indicated nonsignificant increase (P>0.05) with treatment. The concentration of other liver marker compounds and lipid profile were positively affected by the treatment. The values obtained from treated rats showed that, while diesel can induce oxidative damage to the liver, G. latifolium and O. gratissimum in the diet, indicated protective effect against oxidative and cellular degeneration in liver tissues.